[Separation and measurement of lymphocyte functional subpopulations by two color FCM method]

With the popularity of flow cytometry (FCM) and the availability of a wide variety of monoclonal antibodies to human blood cells, analysis of cell surface antigens is now conducted at many facilities as part of their routine testing. Analysis of cell surface antigens by FCM has contributed greatly to the diagnosis and the determination of therapeutic effects in many diseases. In the present study, for detection of lymphocyte surface antigens by two color analysis and selection of appropriate antibodies are described. In addition, the significance of two color analysis is explained by citing hematological neoplasms and several other clinical cases. It has been recently reported that the immunological system is involved in many disease processes. In the future, more detailed studies will be conducted to elucidate the immunological mechanisms in these diseases.