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Related Experiment Videos

Novel core(polyester)-shell(polysaccharide) nanoparticles: protein loading and surface modification with lectins.

J S Rodrigues1, N S Santos-Magalhães, L C B B Coelho

  • 1Universidade Federal de Pernambuco, Departamento de Bioquímica, Laboratório de Imunopatologia Keizo-Asami, Recife, Brazil.

Journal of Controlled Release : Official Journal of the Controlled Release Society
|September 23, 2003
PubMed
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New dextran-PCL nanoparticles were developed for protein delivery. These nanoparticles successfully incorporated proteins and showed potential for targeted oral administration with good cell viability.

Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Polymer Chemistry

Background:

  • Development of novel nanoparticle systems for drug and protein delivery is crucial.
  • Polymeric nanoparticles offer tunable properties for various biomedical applications.

Purpose of the Study:

  • To synthesize and characterize novel dextran-poly(epsilon-caprolactone) (Dex-PCLn) copolymers for nanoparticle formation.
  • To evaluate the efficacy of these nanoparticles for protein incorporation and surface modification with lectins.
  • To assess the potential of these nanoparticles for targeted oral administration and their biocompatibility.

Main Methods:

  • Synthesis of Dex-PCLn block copolymers with varying poly(epsilon-caprolactone) (PCL) block numbers.
  • Nanoparticle formation using two distinct methods and evaluation of particle size.

Related Experiment Videos

  • Incorporation and surface adsorption of model protein (BSA) and lectins (BmoLL, LC) onto nanoparticles.
  • Assessment of surface-bound lectin activity and Caco-2 cell viability.

    • Main Results:

    • Dex-PCLn copolymers successfully formed nanoparticles with a mean diameter of approximately 200 nm.
    • Proteins (BSA, BmoLL, LC) were efficiently incorporated into the nanoparticles.
    • Surface adsorption of Bauhinia monandra lectin (BmoLL) onto nanoparticles retained its hemagglutinating activity.
    • Dex-PCLn nanoparticles demonstrated high Caco-2 cellular viability (>70%) even at high concentrations.

    Conclusions:

    • Novel Dex-PCLn nanoparticles are effective carriers for protein delivery.
    • Surface-modified nanoparticles with lectins show promise for targeted oral administration.
    • The developed nanoparticles exhibit good biocompatibility, suggesting their potential for therapeutic applications.