A high-throughput capillary assay for bacterial chemotaxis
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Updated: Aug 31, 2026

An Improved Chemotaxis Assay for the Rapid Identification of Rhizobacterial Chemoattractants in Root Exudates
Published on: March 25, 2022
Russell Bainer1, Heungwon Park, Philippe Cluzel
1The James Franck Institute and the Institute for Biophysical Dynamics, University of Chicago, 5640 S. Ellis Av., Chicago, IL 60637, USA.
Abstract:
We present a high-throughput capillary assay in order to characterize the chemotactic response of the E. coli bacterium. We measure the number of organisms attracted into an array of 96 capillary tubes containing the attractant L-aspartate. The effect of bacterial concentration on the chemotactic response is reported. Such high-throughput assay can be used to characterize bacterial chemotaxis function of a wide range of biochemical parameters.

