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Related Experiment Videos

Open architecture expression profiling of plant transcriptomes and gene discovery using GeneCalling technology.

Oswald R Crasta1, Otto Folkerts

  • 1CuraGen Corporation, New Haven, CT, USA.

Methods in Molecular Biology (Clifton, N.J.)
|September 23, 2003
PubMed
Summary

GeneCalling technology analyzes gene expression modulation, identifying differentially expressed genes and novel candidates. This genomics tool aids in improving agriculturally important traits in plants.

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Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Genomics tools and bioinformatics have significantly advanced gene expression analysis.
  • Measuring gene expression modulation at the genomic level is now a standard practice.

Purpose of the Study:

  • To introduce and describe the GeneCalling technology for comprehensive gene expression profiling.
  • To highlight the capabilities of GeneCalling in identifying known, novel, and differentially modulated genes.

Main Methods:

  • GeneCalling utilizes an open architecture system to assay over 95% of expressed genes.
  • It employs 96 restriction enzyme pairs to analyze more than 30,000 cDNA fragments for differential modulation.
  • Specific processes like SeqCalling and GeneCall Poisoning are used for gene confirmation and novel gene discovery.

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Main Results:

  • GeneCalling successfully identified differentially modulated cDNA fragments in various plant and fungal species.
  • The technology facilitated the cloning and analysis of unknown and novel genes with significant expression profiles.
  • Application in plant species led to the identification and characterization of genes beneficial for agricultural traits.

Conclusions:

  • GeneCalling offers a powerful, open-architecture approach to gene expression analysis, independent of existing databases.
  • The technology enables the discovery and characterization of both known and novel genes.
  • GeneCalling has demonstrated utility in identifying genes for improving agriculturally important traits in plants.