Fate of salmonellae in citrus oils and aqueous aroma

The manufacture of orange juice sometimes involves the use of flavor fractions recovered from oranges. The impact of such flavor fractions on Salmonella viability was investigated. A five-strain cocktail of salmonellae was challenged with a singlefold cold-pressed peel oil (CPO), a fivefold CPO, a terpeneless CPO, and an aqueous orange aroma stored at 4 and 25 degrees C. The results obtained in this study indicate that the test compounds possess substantial antimicrobial activity and can cause population reductions larger than the 5-log10 performance standard required by the U.S. Food and Drug Administration's juice hazard analysis critical control point rule (21 CFR 120). The times required to achieve 5-log10 reductions in Salmonella populations ranged from 0.03 to 42.8 h. In general, levels of antimicrobial activity for the test substances were in the following order: terpeneless CPO > five-fold CPO > single-fold CPO > aqueous aroma.