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Published on: October 19, 2013
Release by hypoxia of a soluble vasoconstrictor from rabbit small pulmonary arteries
N P Talbot1, P A Robbins, K L Dorrington
1University Laboratory of Physiology, University of Oxford, Parks Road, Oxford OX1 3PT, UK.
Background:
Soluble pulmonary vasoconstrictors released in response to hypoxia have been reported in pig and rat preparations, but not in rabbit preparations.
Methods:
We used myography to evaluate the contribution of a soluble factor to constriction in rabbit small pulmonary arteries (external diameter 300-475 microm) exposed to 45 min hypoxia (PO(2)=9 mm Hg).
Results:
Hypoxia produced gradually intensifying constriction. Return to euoxia (PO(2)=145 mm Hg) for 30 min relaxed only approximately 30% of the constriction, whereas elution of the myograph bath yielded full relaxation. Reapplication of the eluent gradually restored the constriction to its pre-elution level over a 30-min period.
Conclusions:
In this closed system, a soluble factor contributes substantially to hypoxic pulmonary vasoconstriction.
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