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Nonhuman primate parthenogenetic stem cells.

Kent E Vrana1, Jason D Hipp, Ashley M Goss

  • 1Center for Neurobehavioral Study of Alcohol, Department of Physiology and Pharmacology, Wake Forest University School of Medicine, Winston-Salem, NC 27157, USA. kvrana@wfubmc.edu

Proceedings of the National Academy of Sciences of the United States of America
|September 25, 2003
PubMed
Summary
This summary is machine-generated.

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Parthenogenesis enables embryonic development without male contribution. Researchers created pluripotent stem cells from parthenogenetic monkey eggs, demonstrating their potential for cell therapy.

Area of Science:

  • Reproductive Biology
  • Stem Cell Science
  • Developmental Biology

Background:

  • Parthenogenesis, or embryonic development without male contribution, is common in lower organisms but typically fails in mammals.
  • Mammalian parthenotes often do not result in successful pregnancies, limiting their reproductive applications.

Purpose of the Study:

  • To achieve in vitro parthenogenetic development of monkey eggs to the blastocyst stage.
  • To derive and characterize pluripotent stem cells from these parthenogenetic monkey embryos.
  • To evaluate the differentiation potential and therapeutic applications of these novel stem cell lines.

Main Methods:

  • Parthenogenetic activation of Macaca fascicularis eggs in vitro.
  • Isolation and culture of stem cells (Cyno-1 cells) from blastocysts.

Related Experiment Videos

  • Characterization of Cyno-1 cells using stem cell markers, karyotyping, and teratoma formation assays.
  • In vitro differentiation into various cell types and electrophysiological analysis.

    • Main Results:

    • Successful in vitro parthenogenetic development of monkey eggs to the blastocyst stage.
    • Establishment of Cyno-1 cells, a pluripotent stem cell line from parthenogenetic monkey embryos, exhibiting typical human embryonic stem cell markers.
    • Demonstrated differentiation capacity into neurons, cardiomyocytes, smooth muscle, epithelia, and adipocytes.
    • Cyno-1 cells formed teratomas in vivo, confirming pluripotency.
    • Neural precursors derived from Cyno-1 cells acquired functional neuronal characteristics.

    Conclusions:

    • Parthenogenetic activation of nonhuman primate eggs can yield pluripotent stem cells.
    • These stem cells (Cyno-1) possess characteristics similar to human embryonic stem cells.
    • The derived stem cells show potential for autologous cell therapy in females, circumventing the need for competent embryos.