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Simultaneous in situ total internal reflectance fluorescence/atomic force microscopy studies of DPPC/dPOPC

James E Shaw1, Andrea Slade, Christopher M Yip

  • 1Department of Biochemistry, Institute of Biomaterials and Biomedical Engineering, 4 Taddle Creek Road, Toronto, Ontario, Canada, M5S 3G9.

Journal of the American Chemical Society
|September 25, 2003
PubMed

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Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...