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BRCA1 associates with processive RNA polymerase II.

Susan A Krum1, Gustavo A Miranda, Chenwei Lin

  • 1Molecular Biology Institute, The David Geffen School of Medicine at UCLA, Los Angeles, California 90095, USA.

The Journal of Biological Chemistry
|September 25, 2003
PubMed
Summary

The BRCA1 tumor suppressor protein interacts with RNA polymerase II (RNA pol II), a conserved feature across species. This interaction, particularly with processive RNA pol II, is disrupted by DNA damage, suggesting a role in linking transcription and repair.

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • The BRCA1 protein is a crucial tumor suppressor involved in DNA repair and transcriptional regulation.
  • BRCA1 is known to interact with various cellular proteins, including components of the transcriptional machinery.

Purpose of the Study:

  • To investigate the interaction between BRCA1 and RNA polymerase II (RNA pol II) across different species.
  • To determine the functional significance of the BRCA1-RNA pol II interaction in transcription and its regulation by DNA damage.

Main Methods:

  • Utilized GAL4-UAS one-hybrid assays to assess transcriptional activation by BRCA1.
  • Performed fractionation studies to analyze the interaction of BRCA1 with different forms of RNA pol II.
  • Conducted transcriptional run-off assays to evaluate the functionality of BRCA1-RNA pol II complexes.

Main Results:

  • BRCA1-RNA pol II interaction is conserved across species.
  • Full-length BRCA1 proteins do not activate transcription in standard assays; C-terminal activity is poorly conserved.
  • BRCA1 preferentially binds hyperphosphorylated RNA pol II (IIO) over hypophosphorylated RNA pol II (IIA).
  • BRCA1-RNA pol II complexes are functional in transcription and associate with processive RNA pol II in undamaged cells.
  • DNA-damaging agents disrupt the association between BRCA1 and RNA pol II.

Conclusions:

  • BRCA1's interaction with RNA pol II is a conserved mechanism.
  • BRCA1 preferentially interacts with transcriptionally active, processive RNA pol II.
  • The BRCA1-RNA pol II interaction is dynamically regulated by DNA damage, positioning BRCA1 to link transcription and DNA repair.