Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Targeted Cancer Therapies02:57

Targeted Cancer Therapies

8.6K
The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
8.6K
Drugs that Stabilize Microtubules01:15

Drugs that Stabilize Microtubules

2.6K
Microtubules are dynamic structures that undergo cycles of catastrophe and rescue. The microtubules play a central role in cell division by forming the spindle apparatus for segregating the chromosomes. This makes them ideal targets for regulating dividing cells in tumors and malignant cancer cells. Microtubule stabilizing drugs help stabilize the microtubule formation and promote its polymerization. Paclitaxel was the first microtubule stabilizing agent used as anticancer drug in chemotherapy...
2.6K
Combination Therapies and Personalized Medicine02:50

Combination Therapies and Personalized Medicine

5.9K
Combining two or more treatment methods increases the life span of cancer patients while reducing damage to vital organs or tissue from the overuse of a single treatment. Combination therapy also targets different cancer-inducing pathways, thus reducing the chances of developing resistance to treatment.
The combination of the drug acetazolamide and sulforaphane is a good example of combination therapy to treat cancer. The cells in the interior of a large tumor often die due to the hypoxic and...
5.9K
Treatment Resistant Cancers02:56

Treatment Resistant Cancers

3.7K
Cancer is the second leading cause of death in the United States. A cancer cell is genetically unstable and hence can mutate faster. They can also modify their microenvironment and escape immune surveillance. The difficulties in treating cancer are further compounded by the emergence of rapid resistance to anticancer drugs. The most common ways to attain resistance in cancer cells include alteration in drug transport and metabolism, modification of drug target, elevated DNA damage response, or...
3.7K
Drugs that Destabilize Microtubules01:10

Drugs that Destabilize Microtubules

3.6K
Microtubules are dynamic structures and can be regulated by microtubule targeting agents (MTAs). Microtubule destabilizing drugs are a class of MTAs that destabilize and prevent microtubules' polymerization. Both natural and synthetic chemicals can be found under this class of drugs. Vincristine and vinblastine, two vinca alkaloids, and colchicine were among the first to be discovered. These drugs can affect cells in various ways, either by inducing a change in cell morphology, preventing...
3.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<b>Swarming microbombyliids (Diptera, Mythicomyiidae) in amber from Ukraine: a new species and a new synonymy</b>.

Zootaxa·2026
Same author

Decoding motor imagery related to major mimetic muscles from electroencephalography.

Journal of neuroengineering and rehabilitation·2026
Same author

Exploring the Relationship Between Academic Stress and Academic Engagement in Chemistry Laboratory Learning: The Mediating Role of Learning Burnout and the Differentiated Roles of Stress Sources.

Behavioral sciences (Basel, Switzerland)·2026
Same author

Chrysophanol is associated with reduced inflammation and oxidative stress in sepsis-associated acute kidney injury.

Naunyn-Schmiedeberg's archives of pharmacology·2026
Same author

Surface energy-driven perpendicular gradient structure in flexible composite dielectrics for high-temperature capacitive energy storage.

Nature communications·2026
Same author

Mouse Oocyte In Vitro Maturation, Fertilization, and Culture of Preimplantation Embryos.

Journal of visualized experiments : JoVE·2026

Related Experiment Video

Updated: Jan 9, 2026

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes
08:32

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

Published on: May 23, 2025

1.1K

Disrupting Lipid Raft Microdomains to Block Polyploid Giant Cancer Cell Budding and Enhance Radiotherapy Response.

Zheng Deng1,2,3, Haoran Sun1,2, Jin Cheng1,2

  • 1Cancer Center, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 201620, China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|December 2, 2025
PubMed
Summary

Radiotherapy resistance involves cancer cells forming polyploid giant cancer cells (PGCCs) that bud off new cells. Disrupting lipid rafts impairs this budding, enhancing treatment effectiveness and preventing tumor recurrence.

Keywords:
BuddingPolyploid giant cancer cellsRadiotherapyRadio‐sensitizationTumor repopulation

More Related Videos

Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics
06:00

Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics

Published on: May 14, 2016

11.4K
Utilizing Functional Genomics Screening to Identify Potentially Novel Drug Targets in Cancer Cell Spheroid Cultures
07:48

Utilizing Functional Genomics Screening to Identify Potentially Novel Drug Targets in Cancer Cell Spheroid Cultures

Published on: December 26, 2016

11.8K

Related Experiment Videos

Last Updated: Jan 9, 2026

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes
08:32

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

Published on: May 23, 2025

1.1K
Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics
06:00

Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics

Published on: May 14, 2016

11.4K
Utilizing Functional Genomics Screening to Identify Potentially Novel Drug Targets in Cancer Cell Spheroid Cultures
07:48

Utilizing Functional Genomics Screening to Identify Potentially Novel Drug Targets in Cancer Cell Spheroid Cultures

Published on: December 26, 2016

11.8K

Area of Science:

  • Oncology
  • Cell Biology
  • Biochemistry

Background:

  • Radiotherapy failure is often linked to tumor repopulation by resistant cancer cells.
  • Following irradiation, cancer cells can form polyploid giant cancer cells (PGCCs), which are radiation-persistent and can generate progeny via asymmetric budding.

Purpose of the Study:

  • To elucidate the molecular mechanism of PGCC budding after radiation exposure.
  • To identify potential therapeutic strategies targeting PGCCs to improve radiotherapy outcomes.

Main Methods:

  • Identification of the radiation-induced SNCG-FLOT2-CHMP4B signaling axis.
  • Investigation of the role of ASAH1 and SMPD2 in sphingolipid metabolism and FLOT2 in lipid raft-dependent budding.
  • Assessment of the effects of lipid raft disruption using statins or anti-PCSK9 antibodies on PGCC budding and tumor radiosensitivity in vitro and in vivo.

Main Results:

  • A novel SNCG-FLOT2-CHMP4B signaling axis regulates PGCC budding.
  • Sphingolipid metabolism, maintained by ASAH1 and SMPD2, and FLOT2-driven lipid raft germination are crucial for budding.
  • Disruption of lipid rafts impairs PGCC budding, suppresses tumor repopulation, and enhances radiosensitivity.
  • Clinically available lipid-lowering agents show potential as radiosensitizers.

Conclusions:

  • A conserved membrane remodeling program underlies PGCC budding.
  • Targeting lipid rafts represents a promising therapeutic strategy to prevent tumor recurrence after radiotherapy.
  • Lipid-lowering agents could serve as innovative radiosensitizers to improve radiotherapy efficacy.