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Potyviruses, chaos or order?

L Bos1

  • 1Research Institute for Plant Protection (IPO-DLO), Wageningen, The Netherlands.

Archives of Virology. Supplementum
|January 1, 1992
PubMed
Summary

Classifying potyviruses is challenging due to significant biological variation. New serological methods targeting coat protein N-termini show promise but have exceptions, questioning their sufficiency for definitive virus classification.

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Further Evidence that Shallot Yellow Stripe Virus (SYSV) Is a Distinct Potyvirus and Reidentification of Welsh Onion Yellow Stripe Virus as a SYSV Strain.

Phytopathology·2008

Area of Science:

  • Plant Virology
  • Molecular Biology
  • Taxonomy

Background:

  • Potyviruses were initially distinguished by limited host range and distinct biological/serological properties.
  • Increased knowledge revealed wider host ranges and biological overlap, leading to the 'continuum hypothesis' for potyviruses.

Purpose of the Study:

  • To evaluate the effectiveness of new serological methods for potyvirus classification.
  • To determine if coat protein N-terminal epitopes are reliable criteria for distinguishing potyvirus strains and species.

Main Methods:

  • Review of historical and recent studies on potyvirus characterization.
  • Analysis of serological cross-reactivity using polyclonal antisera and antibodies to coat protein N-termini.
  • Comparison of serological data with biological properties (host range, inclusion bodies, coat protein migration).

Main Results:

  • Traditional methods and nucleic acid hybridization failed to unambiguously discriminate between potyviruses.
  • Antibodies to N-terminal epitopes offered improved discrimination but showed exceptions.
  • Bean yellow mosaic virus and clover yellow vein virus reacted with shared N-terminal antibodies despite distinct biological differences.

Conclusions:

  • Coat protein N-terminal serology presents a potential tool for potyvirus classification but is not universally definitive.
  • The biological and genomic diversity of potyviruses challenges single-criterion classification systems.
  • Further research is needed to refine classification criteria for potyviruses, considering both serological and biological data.

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