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Stretching of single DNA molecules by LB technique for restriction site mapping
Kuniharu Ijiro1, Yasutaka Matsuo, Masatsugu Shimomura
1PRESTO, Japan Science and Technology Corporation, 4-1-8, Honcho, Kawaguchi, Saitama 332-0012, Japan.
Nucleic Acids Research. Supplement (2001)
|September 27, 2003
Abstract:
Stretched single DNA molecules were prepared on substrates by Langmuir-Blodgett (LB) method for the restriction site mapping. Stretched single Lambda DNA and EcoRI bound to the DNA were observed by scanning near-field optical microscope (SNOM). As a result, the specific binding sites of EcoRI on the Lambda DNA molecules were determined.