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Updated: Aug 31, 2026

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
Published on: April 3, 2014
Efficient cross-linking reactions by functional nucleobases capable of in situ activation under neutral conditions
Fumi Nagatsugi1, Yasushi Suenaga, Shigeki Sasaki
1Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Abstract:
We have previously described that the oligonucleotides containing phenylsulfide derivatives of 2-amino-6-vinylpurine nucleoside analog are activated within duplex to form cross-link toward cytidine selectively at the target site. However, the cross-linking reaction via the in-situ activation needs acidic conditions. In this paper, we wish to report that the new 2-amino-6-vinylpurine derivatives designed to have a structure of "double-activation" for in-situ activation exhibit fast cross-linking with cytidine under neutral conditions.
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