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Updated: Aug 19, 2026

Multiplex PCR and Reverse Line Blot Hybridization Assay (mPCR/RLB)
Published on: August 6, 2011
Specificity of one-base mismatch detection with MagiProbe
1Institute for Medical Research, Wakunaga Pharmaceutical Co., Ltd., 1624 Shimokotachi, Kodacho, Takatagun, Hiroshima 739-1195, Japan.
Abstract:
MagiProbe have been demonstrated to be a homogeneous type of probe that emits fluorescence upon hybridization and with specificity for one-base mismatch. To obtain more comprehensive information for the properties of mismatch recognition, examination of mismatch recognition of MagiProbe for the eight single-base mismatches in variety of sequence contexts was performed. The results indicate that the recognition of base mismatches is influenced predominantly by combination of mispaired bases.
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