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Related Experiment Videos

Design of a ribonucleopeptide biosensor.

Masaki Hagihara1, Akiyoshi Hirata, Katsutoshi Ohkubo

  • 1Institute of Advanced Energy, Kyoto University, Uji, Kyoto 611-0011, Japan.

Nucleic Acids Research. Supplement (2001)
|September 27, 2003
PubMed
Summary

Researchers designed ATP-binding ribonucleopeptide receptors and converted them into sensors. The novel sensor shows a significant fluorescence change when adenosine triphosphate (ATP) is present.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • Adenosine triphosphate (ATP) is a crucial molecule in cellular energy transfer.
  • Ribonucleopeptide receptors are essential for regulating cellular processes.
  • Developing specific sensors for biomolecules like ATP is vital for research and diagnostics.

Purpose of the Study:

  • To design and develop novel ribonucleopeptide receptors capable of binding adenosine triphosphate (ATP).
  • To engineer these ATP-binding receptors into functional ribonucleopeptide sensors.
  • To validate the sensing capability of the developed system through fluorescence detection.

Main Methods:

  • Structure-based design was employed to create the initial ribonucleopeptide receptors.
  • In vitro selection methods were utilized to optimize ATP binding affinity and specificity.

Related Experiment Videos

  • A fluorophore was incorporated into the peptide subunit to create a fluorescent sensor.
  • Fluorescence intensity changes were measured upon the addition of ATP.

    • Main Results:

    • The designed ribonucleopeptide receptors demonstrated submillimolar affinity for ATP.
    • These receptors effectively discriminated ATP from other ribonucleotides.
    • The fluorophore-labeled ribonucleopeptide complex exhibited a significant change in fluorescence intensity in the presence of ATP.
    • This indicates the successful conversion of a binding receptor into a functional sensor.

    Conclusions:

    • A straightforward strategy was developed to create a ribonucleopeptide sensor for ATP.
    • The sensor exhibits high sensitivity and specificity for ATP detection.
    • This work provides a new tool for monitoring ATP levels in biological systems.