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Enzyme cascade reactions encapsulated in a liposome compartment with size-limited molecular transport
Shiwei Zhang1, Peng Lin1, Futa Komatsubara1
1Institute of Advanced Energy, Kyoto University, Uji, Kyoto 611-0011, Japan.
Abstract:
We designed a liposome-coated DNA origami compartment to encapsulate a defined number of cascade enzymes with retained cofactors. Confinement enhanced cascade reaction efficiency particularly at low cofactor levels. Efficiency gains plateaued at higher NADH concentrations, indicating that intermediate diffusion and enzyme spatial organization influence cascade performance in confined environments.
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