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The dog genome: survey sequencing and comparative analysis
Ewen F Kirkness1, Vineet Bafna, Aaron L Halpern
1The Institute for Genomic Research, Rockville, MD 20850, USA.
Science (New York, N.Y.)
|September 27, 2003
Summary
Dog genome sequencing reveals its utility for comparative genomics and identifying genetic traits. This resource aids in understanding mammalian evolution and canine disease genetics.
Area of Science:
- Comparative genomics
- Mammalian genetics
- Canine genome sequencing
Background:
- The dog genome sequence provides a valuable resource for comparative analysis.
- Understanding mammalian genome evolution requires cross-species genomic data.
Purpose of the Study:
- To demonstrate the power of sample sequencing for comparative mammalian genomics.
- To generate species-specific canine genomic resources.
- To identify genetic elements for disease and trait mapping in dogs.
Main Methods:
- Survey sequencing of the dog genome (6.22 million reads, 1.5x coverage).
- Alignment of dog sequence reads to the human genome.
- Comparative analysis of mutation rates, synteny, repeat content, and phylogeny across species.
Main Results:
- Over 650 million base pairs of dog sequence uniquely align to the human genome.
- Putative orthologs for 18,473 human genes were identified in the dog genome.
- Identification of various polymorphic elements within the dog genome.
Conclusions:
- Dog genome sequencing is a powerful tool for comparative genomics.
- The generated canine genome data is crucial for understanding mammalian evolution.
- Polymorphic elements identified will facilitate mapping the genetic basis of canine diseases and traits.
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