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Updated: Aug 30, 2026

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
Published on: June 21, 2018
Prospective study of the association between the proline to alanine codon 12 polymorphism in the PPARgamma gene and
Asli Memisoglu1, Frank B Hu, Susan E Hankinson
1Department of Epidemiology, Harvard School of Public Health, Boston, Massachusetts 02115, USA. amemisog@hsph.harvard.edu
Objective:
To determine whether the Pro12Ala polymorphism in the PPARgamma gene was associated with risk of type 2 diabetes in the Nurses' Health Study.
Research Design And Methods:
The study was a nested case-control study of 387 incident cases of type 2 diabetes and 771 matching control subjects nested within the Nurses' Health Study, a prospective cohort study. Association between PPARgamma genotype and incident type 2 diabetes was estimated using logistic regression.
Results:
Carriers of the PPARgamma variant 12Ala allele had reduced risk of type 2 diabetes compared with noncarriers. Unadjusted and adjusted odds ratios of type 2 diabetes were 0.74 (95% CI 0.55-1.00) and 0.72 (0.52-0.99), respectively.
Conclusions:
The results of this study provide further support for an inverse association between the PPARgamma variant 12Ala allele and risk of type 2 diabetes.
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