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Updated: Aug 30, 2026

High-resolution Tandem Mass Spectrometry for Studying Chemical Constituents of Gynura bicolor DC
Published on: February 2, 2024
[Simultaneous determination of 3 kinds of components in Gardenia by high-performance liquid chromatography under
Yan Xu1, Jin Cao, Yi-ming Wang
1Institute of Medicine, Tsinghua University, Beijing 100084, China.
Aim:
To determine 3 kinds of biologically active components (9 compounds) in Gardenia, namely geniposidic acid, gentiobioside, gardenoside, genipin geniposide, chlorogenic acid, crocin 1, crocin 2, crocin 3 and crocetin simultaneously and quantitatively.
Methods:
An HPLC method detected by 3 different UV-vis waves--240 nm for geniposidic acid, geniposide, gardenoside, genipin gentiobioside, 330 nm for chlorogenic acid, 440 nm for crocin 1, crocin 2, crocin 3 and crocetin respectively, has been developed: Phenomenex Luna C18 column(250 mm x 4.6 mm ID, 5 microns) with linear gradient elution using methanol-acetotrile (9:1) and 0.3% aqueous formic acid.
Results:
This method successfully determined 9 compounds in Gardenia (from 5 different areas) simultaneously and quantitatively.
Conclusion:
This is a more reasonable and credible quality control method for the Chinese traditional medicine.
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