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Updated: Aug 30, 2026

Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds
Published on: November 12, 2016
Determination of the triacylglycerol composition of coffee beans by reverse-phase high-performance liquid
Gulab N Jham1, Boryana Nikolova-Damyavova, Mirtes Viera
1Universidade Federal de Viçosa, Labarótorio de Pesquisas de Produtos Naturais, Departamento de Química, Viçosa, MG 36.570-000, Brazil. gulab@ufv.br
Abstract:
Reverse-phase HPLC with refractive index and light scattering detectors in isocratic and gradient elution modes, respectively, was applied for the separation of the major triacylglycerols (TAG) in coffee lipids. Twelve TAG species could be identified and determined using a linear gradient of acetonitrile in dichloromethane: dichloroethane. The quantitative evaluation was based on the relative area percentages derived directly from a data-station. The procedure was applied to determine the TAG composition of three types of coffee beans harvested in two coffee producing areas in Brazil and dried by two commonly used procedures. No significant differences in the TAG compositions due to the type, origin and drying procedure were found.
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