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Related Experiment Videos

Efficient gene modulation in mouse epiblast using a Sox2Cre transgenic mouse strain.

Shigemi Hayashi1, Paula Lewis, Larysa Pevny

  • 1Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA.

Mechanisms of Development
|October 1, 2003
PubMed
Summary

A new transgenic mouse line, Sox2Cre, enables precise gene manipulation in the epiblast, crucial for early embryonic development. This tool offers more efficient and specific gene knockout than previous methods, aiding developmental studies.

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Area of Science:

  • Developmental Biology
  • Genetics
  • Molecular Biology

Background:

  • The Sox2 gene plays a critical role in early embryonic development.
  • Understanding gene function in specific embryonic tissues requires precise genetic tools.

Purpose of the Study:

  • To generate and characterize a novel transgenic mouse line (Sox2Cre) for epiblast-specific gene manipulation.
  • To compare the efficiency and specificity of Sox2Cre with existing Cre lines.

Main Methods:

  • Generation of a transgenic mouse line expressing Cre recombinase under the Sox2 promoter.
  • Utilizing a R26R reporter line to assess Cre activity.
  • Crossing Sox2Cre mice with conditional Sonic hedgehog (Shh) alleles to evaluate gene knockout efficacy.

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Main Results:

  • Sox2Cre mediates recombination in all epiblast cells by embryonic day 6.5, with minimal activity in extraembryonic tissues.
  • Sox2Cre;Shh conditional embryos exhibit phenotypes identical to Shh null mutants.
  • Sox2Cre demonstrates more efficient and earlier recombination compared to the Mox2Cre (MORE) line, particularly at gastrulation stages.

Conclusions:

  • The Sox2Cre line provides a powerful tool for complete, epiblast-specific gene inactivation in mouse embryos.
  • Sox2Cre is more effective than the MORE line for generating null phenotypes.
  • The mosaic activity of the MORE line can be useful for studying partial gene loss-of-function phenotypes.