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Related Experiment Videos

An in vitro model of angiogenesis: basic features.

E T Bishop1, G T Bell, S Bloor

  • 1BioCuRe Ltd, Ellon, Aberdeenshire, UK.

Angiogenesis
|October 1, 2003
PubMed
Summary
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Researchers developed a novel in vitro model for angiogenesis using co-cultures of endothelial cells and fibroblasts. This model effectively quantifies agents that stimulate or inhibit blood vessel formation, validating its relevance to in vivo processes.

Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Vascular Biology

Background:

  • Angiogenesis, the formation of new blood vessels, is crucial for development and disease.
  • Existing in vitro models often require complex conditions or additional growth factors.
  • A simplified, quantifiable model is needed to study angiogenesis and test therapeutic agents.

Purpose of the Study:

  • To establish and validate a robust in vitro model of angiogenesis.
  • To demonstrate the model's utility in quantifying angiogenic stimulators and inhibitors.
  • To confirm the biological relevance of the model to in vivo angiogenesis.

Main Methods:

  • Co-culturing human umbilical vein endothelial cells (HUVEC) with human diploid fibroblasts.
  • Utilizing standard endothelial growth medium (EGM) with low fetal calf serum (2%).

Related Experiment Videos

  • Stimulating angiogenesis with vascular endothelial growth factor (VEGF) and inhibiting it with anti-VEGF antibodies or suramin.
  • Quantifying results using image analysis, confocal microscopy, electron microscopy, and immunochemistry (PECAM-1 expression).

    • Main Results:

    • The co-culture system successfully modeled angiogenesis without additional growth factors or matrix proteins.
    • VEGF stimulation led to the formation of microvasculature-like structures.
    • Inhibition was achieved using anti-VEGF antibodies and suramin.
    • Image analysis provided rapid quantification of stimulatory and inhibitory effects.
    • Microscopic and immunochemical analyses confirmed tubule formation and endothelial cell marker expression (PECAM-1).

    Conclusions:

    • The developed co-culture system serves as a validated in vitro model for angiogenesis.
    • This model is suitable for quantifying the effects of various agents on blood vessel formation.
    • The system offers a reliable platform for screening drugs and understanding angiogenic processes in vitro.