Separation and determination synchronously by multichannel mode-filtered light capillary electrochromatography
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Updated: Aug 30, 2026

Automated HPLC Separation Using LC-Mate: An Integrated Repetitive Autosampler and Fraction Collector for Microscale Purification
Published on: February 27, 2026
Xinbing Zuo1, Kemin Wang, Leiji Zhou
1State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Institute of Biological Technology, Hunan University, Changsha, P. R. China.
Abstract:
Mode-filtered light capillary electrophoresis (CE) was developed for capillary electrochromatography (CEC) by applying annular columns of different size (250, 320, and 530 micro m) and a bovine serum albumin (BSA)-modified chiral stationary phase. BSA was encapsulated on the pretreated surface of an optic fiber and the inner wall of a capillary using sol-gel technique with a resultant larger active stationary phase area. The coating was examined by atomic force microscopy (AFM) and Fourier transform infrared (FT-IR). The separation of DL-tryptophan (10 mM) was investigated under different voltage/current conditions.
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