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Quantification of neutral red pinocytosis by small numbers of adherent cells: comparative studies
B Płytycz1, M Rózanowska, R Seljelid
1Laboratory of Evolutionary Immunology, Jagiellonian University, Kraków, Poland.
Folia Biologica
|January 1, 1992
Abstract:
A colorimetric assay has been developed for studies on neutral red (NR) pinocytosis by small numbers (below 2 x 10(5)) of adherent cells cultured in 96 well plates. The NR uptake per cell mass was much higher in the sea urchin perivisceral adherent cells and human HL-60 cell line monolayers than in the murine and Atlantic salmon macrophages. The apparent difference points to the usefulness of this novel assay in comparative studies.