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Isolation and characterization of phenol-catabolizing bacteria from a coking plant
Wael S El-Sayed1, Mohamed K Ibrahim, Mohamed Abu-Shady
1Microbiology Department, Faculty of Science, Ain Shams University, Cairo, Egypt.
Bioscience, Biotechnology, and Biochemistry
|October 2, 2003
Abstract:
New phenol degrading bacteria with high biodegradation activity and high tolerance were isolated as Burkholderia cepacia PW3 and Pseudomonas aeruginosa AT2. Both isolates could grow aerobically on phenol as a sole carbon source even at 3 g/l. The whole-cell kinetic properties for phenol degradation by strains PW3 and AT2 showed a Vmax of 0.321 and 0.253 mg/l/min/(mg protein), respectively. The metabolic pathways for phenol biodegradation in both strains were assigned to the meta-cleavage activity of catechol 2,3-dioxygenase.