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Determination of picogram levels of heptylphysostigmine in human plasma using high-performance liquid chromatography
M L Herold1, M L Constanzer, B K Matuszewski
1Merck Sharp and Dohme Research Laboratories, West Point, PA 19486.
Journal of Chromatography
|October 23, 1992
Abstract:
A sensitive (50 pg/ml) method for the determination of heptylphysostigmine in human plasma is described. The procedure is based on liquid-liquid extraction of the drug from buffered plasma, and analysis of the concentrated organic extract using high-performance liquid chromatography on a silica column, under normal-phase chromatographic conditions, with fluorescence detection. Physostigmine was used as an internal standard. The assay has been fully validated in the concentration range 50-2000 pg/ml and utilized for the analysis of clinical samples from subjects dosed with heptylphysostigmine.