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Synthesis of CCK-8 tetrapeptide fragment by enzymatic method
Guangya Xiang1, Heiner Eckstein
1Pharmacy School, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.
Abstract:
The enzymatic synthesis of a tetrapeptide Phac-Met-Gly-Trp-Met-OEt, a fragment of the cholecystokinin C-terminal octapeptide CCK-8, was reported. This fragment was synthesized by coupling Phac-Met-OEt with Gly-OMe, Trp-OMe and Met-OEt successively. These three steps were catalyzed by alpha-chymotrpsin, Papain and alpha-chymotrpsin respectively. The results of FAB-MS showed that all the products had the correct molecular mass.