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Updated: Aug 30, 2026

One-step Extraction and Zymographic Analysis of Bacterial Gelatinases
Published on: August 1, 2025
[The extracellular proteases of the phytopathogenic bacterium Xanthomonas campestris]
E E Kalashnikova1, M P Chernyshova, V V Ignatov
1Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, pr. Entuziastov 13, Saratov, 410015 Russia.
Abstract:
The culture liquids of three Xanthomonas campestris pv. campestris strains were found to possess proteolytic activity. The culture liquid of strain B-611 with the highest proteolytic activity was fractionated by salting-out with ammonium sulfate, gel filtration, and ion-exchange chromatography. The electrophoretic analysis of active fractions showed the presence of two proteases in the culture liquid of strain B-611, the major of which being serine protease. The treatment of cabbage seedlings with the proteases augmented the activity of peroxidase in the cabbage roots by 28%.
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