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Related Experiment Video

Updated: Jun 30, 2026

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy

Published on: June 27, 2014

Single-molecule spectroscopy of interfacial electron transfer.

Michael W Holman¹, Ruchuan Liu, David M Adams

¹Department of Chemistry, Columbia University, 3000 Broadway, New York, New York 10027, USA.

Journal of the American Chemical Society

|October 9, 2003

Summary

Single-molecule spectroscopy reveals electron transfer rates in molecular systems. This technique measures photoinduced electron transfer and back electron transfer, crucial for molecular electronics.

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Novel Techniques for Observing Structural Dynamics of Photoresponsive Liquid Crystals

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Area of Science:

Physical Chemistry
Materials Science
Nanotechnology

Background:

Single-molecule spectroscopy (SMS) offers insights into individual molecular properties, overcoming ensemble averaging limitations.
Photoinduced interfacial electron transfer (IET) is fundamental to many chemical and biological processes.
Understanding electron transfer dynamics is key for developing molecular electronic devices.

Purpose of the Study:

To utilize SMS for quantifying photoinduced interfacial electron transfer (IET) and back electron transfer rates.
To investigate a prototypical chromophore-bridge-electrode nonadiabatic electron transfer system.
To demonstrate the applicability of SMS in studying electron transfer dynamics in complex molecular systems.

Main Methods:

Synthesis of N-(1-hexylheptyl)-N'-(12-carboxylicdodecyl)perylene-3,4,9,10-tetracarboxylbisimide.
Formation of mixed self-assembled monolayers (SAMs) on a tin-doped indium oxide (ITO) electrode.
Analysis of single-molecule fluorescence time trajectories, identifying "blinks" as electron transfer events.

Main Results:

Observed "blinks" in fluorescence, attributed to electron injection and charge recombination.
Measured forward and back electron transfer rates in the millisecond to second range.
Demonstrated that fluorescence trajectories for individual molecules fit single exponential kinetics for electron transfer events.

Conclusions:

SMS provides a powerful method to study electron transfer dynamics at the single-molecule level.
The methodology is versatile for investigating various factors influencing electron transfer rates (distance, orientation, etc.).
Findings have significant implications for the design and control of molecular electronic devices.