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Related Experiment Videos

Membrane fluidity adjustments in ethanol-stressed Oenococcus oeni cells.

M Graça Da Silveira1, Elena A Golovina, Folkert A Hoekstra

  • 1Wageningen University and Research Center, 6700 EV Wageningen, The Netherlands.

Applied and Environmental Microbiology
|October 9, 2003
PubMed
Summary

Oenococcus oeni cells adapt to ethanol by altering their cytoplasmic membrane fluidity. This adaptation reduces ethanol-induced leakage, enhancing cell survival in alcoholic environments.

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Area of Science:

  • Microbiology
  • Biochemistry
  • Cell Biology

Background:

  • Ethanol tolerance is crucial for microorganisms in fermented beverages.
  • Cytoplasmic membrane integrity is vital for cell survival under stress.

Purpose of the Study:

  • Investigate ethanol's effect on Oenococcus oeni cytoplasmic membrane fluidity.
  • Determine the role of membrane changes in acquired ethanol tolerance.

Main Methods:

  • Electron spin resonance spectroscopy using doxyl-stearic acids to measure membrane fluidity.
  • Carboxyfluorescein (cF) leakage assay to assess membrane integrity.
  • Analysis of membrane lipid composition.

Main Results:

  • Ethanol increased membrane fluidity; adaptation to ethanol decreased fluidity.

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  • Adapted cells showed reduced carboxyfluorescein leakage.
  • Ethanol adaptation involved increased fatty acid unsaturation and decreased total lipids.
  • Short-term adaptation occurred without de novo protein synthesis.

    • Conclusions:

    • Oenococcus oeni adjusts cytoplasmic membrane fluidity to tolerate ethanol.
    • Decreased membrane fluidity at the lipid-water interface protects against ethanol-induced leakage.
    • Physicochemical modification of the membrane is key to acquired ethanol tolerance.