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Mitochondrial oxidative substrate selection in porcine bladder smooth muscle
Christopher D Hardin1, Brian D Kleiber, Tina M Roberts
1Department of Physiology, MA-415 Medical Sciences Building, University of Missouri-Columbia, Columbia, MO 65212, USA.
The Journal of Urology
|October 9, 2003
Summary
Bladder smooth muscle (BSM) can adapt its metabolism but may be susceptible to lipotoxicity from high-fat diets, potentially impairing detrusor function.
Area of Science:
- Cellular metabolism
- Physiology
- Biochemistry
Background:
- High-fat diets are prevalent, potentially impacting bladder smooth muscle (BSM) metabolism.
- Understanding BSM's metabolic flexibility is crucial for assessing lipotoxicity risks.
Purpose of the Study:
- To investigate BSM's mitochondrial substrate selection patterns.
- To determine BSM's adaptability to altered metabolic substrate availability.
- To assess BSM's susceptibility to lipotoxicity.
Main Methods:
- BSM strips incubated with varying concentrations of labeled glucose and acetate.
- Metabolite analysis using 13C-nuclear magnetic resonance (NMR).
- BSM organ culture for 4 days to observe phenotypic changes.
Main Results:
- BSM utilizes both glucose and acetate, with approximately 50% from glucose at physiological levels.
- BSM increases acetate utilization and spares glucose/lipids at elevated acetate concentrations (up to 0.5 mM).
- Prolonged culture leads to metabolic adaptation, favoring intracellular substrates and reduced glucose use, mimicking hyperlipidemia.
Conclusions:
- BSM exhibits moderate plasticity in mitochondrial substrate selection.
- Excessive lipid availability may induce lipotoxicity in BSM.
- Lipotoxicity in BSM could lead to impaired detrusor function.