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A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence (FUEL)
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Regiodependent luminescence quenching of biotinylated N-sulfonyl-acridinium-9-carboxamides by avidin

Maciej Adamczyk1, Phillip G Mattingly, Jeffrey A Moore

  • 1Department of Chemistry (D09MD), Diagnostics Division, Abbott Laboratories, 100 Abbott Park Road, AP20, Abbott Park, Illinois 60064-6016, USA. maciej.adamczyk@abbot.com

Organic Letters
|October 11, 2003
PubMed

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Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...