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Related Experiment Videos

Urogenital inflammation: changes of leucocytes and ROS.

R Henkel1, G Maass, M Hajimohammad

  • 1Centre of Dermatology and Andrology, Justus Liebig University, Giessen, Germany. ralf.henkel@derma.med.uni-giessen.de

Andrologia
|October 11, 2003
PubMed
Summary
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Quantifying excess white blood cells (leukocytes) in semen is crucial for diagnosing male infertility. Different detection methods yield varying results, impacting clinical interpretation and understanding of leukocyte effects on sperm function and reproductive outcomes.

Area of Science:

  • Reproductive Medicine
  • Andrology
  • Semen Analysis

Background:

  • Excess leukocytes in semen (leukocytospermia) are linked to male infertility and genital tract infections.
  • Current WHO guidelines define leukocytospermia but lack standardized quantification methods.
  • Discrepancies exist between different methods for detecting leukocytes in semen.

Purpose of the Study:

  • To compare different methods for quantifying leukocytes in semen.
  • To investigate the correlation between leukocyte counts and sperm parameters.
  • To assess the impact of leukocytes on sperm function and in vitro fertilization (IVF) outcomes.

Main Methods:

  • Comparison of peroxidase staining with monoclonal antibodies (CD15, CD45, CD68) for leukocyte detection.

Related Experiment Videos

  • Correlation analysis of leukocyte counts with sperm concentration, morphology, and acrosome reaction.
  • Evaluation of leukocyte markers (PMN elastase, round cells, peroxidase-positive cells) in relation to IVF fertilization and pregnancy rates.
  • Assessment of sperm DNA fragmentation using TUNEL assay.

    • Main Results:

    • Significant differences were observed between peroxidase staining and CD45 antibody methods for detecting leukocytospermia.
    • Peroxidase-positive cell concentration correlated with polymorphonuclear granulocyte (PMN) elastase.
    • Leukocytes negatively impacted acrosome reaction inducibility.
    • No correlation was found between leukocyte markers and IVF fertilization or pregnancy rates.
    • Sperm DNA fragmentation (TUNEL assay) negatively correlated with pregnancy rates, suggesting a potential role of reactive oxygen species (ROS) from leukocytes.

    Conclusions:

    • Standardization of semen leukocyte quantification methods is needed due to significant discrepancies.
    • Leukocytes may have a detrimental effect on sperm function, particularly acrosome reaction.
    • While direct correlation with IVF outcomes was not observed, sperm DNA fragmentation, potentially influenced by leukocytes, impacts pregnancy rates.