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[A new R-plasmid from Yersinia pseudotuberculosis]
Summary
A novel R-plasmid, pLD140, was identified in Yersinia pseudotuberculosis strain 140-P. This plasmid confers resistance to streptomycin, tetracycline, and sulfonamides, belonging to incompatibility group IncP.
Area of Science:
- Microbiology
- Molecular Biology
- Genetics
Context:
- Yersinia pseudotuberculosis is an important bacterial pathogen.
- R-plasmids play a crucial role in the spread of antibiotic resistance.
- Characterization of novel R-plasmids is essential for understanding resistance mechanisms.
Purpose:
- To isolate and characterize a novel R-plasmid from Yersinia pseudotuberculosis strain 140-P.
- To determine the genetic elements responsible for antibiotic resistance.
- To investigate the plasmid's incompatibility group and restriction endonuclease profile.
Summary:
- A new R-plasmid, designated pLD140, was isolated from Yersinia pseudotuberculosis strain 140-P, found in Far East soil.
- This plasmid is conjugation proficient and confers cellular resistance to streptomycin, tetracycline, and sulfonamides.
- pLD140 belongs to incompatibility group IncP and exhibits distinct BamHI and SalI restriction endonuclease profiles compared to RP4 family plasmids.
Impact:
- Discovery of a new R-plasmid contributing to antibiotic resistance in Yersinia pseudotuberculosis.
- Provides insights into plasmid diversity and evolution.
- Potential implications for tracking and controlling the spread of antibiotic resistance genes.
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