Related Experiment Video

Updated: Aug 7, 2026

'Bioluminescent' Reporter Phage for the Detection of Category A Bacterial Pathogens
11:31

'Bioluminescent' Reporter Phage for the Detection of Category A Bacterial Pathogens

Published on: July 8, 2011

[A new R-plasmid from Yersinia pseudotuberculosis]

A V Drozdov, T V Belokurova, T S Il'ina

    Molekuliarnaia Genetika, Mikrobiologiia I Virusologiia
    |May 1, 1992
    PubMed

    Insights

    A novel R-plasmid, pLD140, was identified in Yersinia pseudotuberculosis strain 140-P. This plasmid confers resistance to streptomycin, tetracycline, and sulfonamides, belonging to incompatibility group IncP.

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genetics

    Context:

    • Yersinia pseudotuberculosis is an important bacterial pathogen.
    • R-plasmids play a crucial role in the spread of antibiotic resistance.
    • Characterization of novel R-plasmids is essential for understanding resistance mechanisms.

    Purpose:

    • To isolate and characterize a novel R-plasmid from Yersinia pseudotuberculosis strain 140-P.
    • To determine the genetic elements responsible for antibiotic resistance.
    • To investigate the plasmid's incompatibility group and restriction endonuclease profile.

    Summary:

    • A new R-plasmid, designated pLD140, was isolated from Yersinia pseudotuberculosis strain 140-P, found in Far East soil.
    • This plasmid is conjugation proficient and confers cellular resistance to streptomycin, tetracycline, and sulfonamides.
    • pLD140 belongs to incompatibility group IncP and exhibits distinct BamHI and SalI restriction endonuclease profiles compared to RP4 family plasmids.

    Impact:

    • Discovery of a new R-plasmid contributing to antibiotic resistance in Yersinia pseudotuberculosis.
    • Provides insights into plasmid diversity and evolution.
    • Potential implications for tracking and controlling the spread of antibiotic resistance genes.

    More Related Videos

    Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis10:33

    Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis

    Published on: June 17, 2019

    Quantifying Yersinia pseudotuberculosis Type III Secretion System Activity Following Iron Starvation and Anaerobic Growth08:36

    Quantifying Yersinia pseudotuberculosis Type III Secretion System Activity Following Iron Starvation and Anaerobic Growth

    Published on: May 31, 2024

    Related Concept Videos

    Plasmids01:28

    Plasmids

    Plasmids are extrachromosomal DNA molecules found in bacteria, archaea, and some eukaryotic microbes like yeast. These small, circular DNA structures typically contain fewer than 30 genes, although some may exist linearly. Plasmids vary in their number within a cell, known as copy number. Single-copy plasmids are present in one copy per cell and multi-copy plasmids are present in multiple copies, reaching over 100 copies per cell.Plasmids usually replicate independently of the chromosomal DNA...
    DNA Bacteriophages01:26

    DNA Bacteriophages

    Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
    Plague01:24

    Plague

    Plague is a highly virulent zoonotic disease caused by Yersinia pestis, a Gram-negative, facultatively anaerobic coccobacillus. This pathogen primarily circulates among rodent populations and is transmitted to humans through the bite of infected fleas. Additional transmission routes include direct contact with infected animal tissue or inhalation of respiratory droplets from individuals with pneumonic plague. These multiple transmission pathways highlight the bacterium’s potential for rapid...