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Related Experiment Videos

[A new R-plasmid from Yersinia pseudotuberculosis].

A V Drozdov, T V Belokurova, T S Il'ina

    Molekuliarnaia Genetika, Mikrobiologiia I Virusologiia
    |May 1, 1992
    PubMed
    Summary

    A novel R-plasmid, pLD140, was identified in Yersinia pseudotuberculosis strain 140-P. This plasmid confers resistance to streptomycin, tetracycline, and sulfonamides, belonging to incompatibility group IncP.

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    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genetics

    Context:

    • Yersinia pseudotuberculosis is an important bacterial pathogen.
    • R-plasmids play a crucial role in the spread of antibiotic resistance.
    • Characterization of novel R-plasmids is essential for understanding resistance mechanisms.

    Purpose:

    • To isolate and characterize a novel R-plasmid from Yersinia pseudotuberculosis strain 140-P.
    • To determine the genetic elements responsible for antibiotic resistance.
    • To investigate the plasmid's incompatibility group and restriction endonuclease profile.

    Summary:

    • A new R-plasmid, designated pLD140, was isolated from Yersinia pseudotuberculosis strain 140-P, found in Far East soil.
    • This plasmid is conjugation proficient and confers cellular resistance to streptomycin, tetracycline, and sulfonamides.

    Related Experiment Videos

  • pLD140 belongs to incompatibility group IncP and exhibits distinct BamHI and SalI restriction endonuclease profiles compared to RP4 family plasmids.
  • Impact:

    • Discovery of a new R-plasmid contributing to antibiotic resistance in Yersinia pseudotuberculosis.
    • Provides insights into plasmid diversity and evolution.
    • Potential implications for tracking and controlling the spread of antibiotic resistance genes.