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Monoclonal antibody technology for mycotoxins
A A Candlish1, J E Smith, W H Stimson
1May & Baker Diagnostics Ltd., Montrose House, Glasgow, UK.
Biotechnology Advances
|January 1, 1989
Summary
Monoclonal antibodies (MABs) offer sensitive and reliable detection of mycotoxins in food and animal products. These immunoassays simplify sample preparation for rapid analysis of toxins like aflatoxins and ochratoxin A.
Area of Science:
- Analytical Chemistry
- Immunology
- Food Safety
Background:
- Mycotoxins pose significant risks to human and animal health.
- Current detection methods can be complex and time-consuming.
- Hybridoma technology enables the development of specific antibodies.
Purpose of the Study:
- To review the development and application of monoclonal antibodies (MABs) for mycotoxin detection.
- To highlight the advantages of MABs in immunological assays.
- To discuss the use of MABs in various mycotoxin analysis techniques.
Main Methods:
- Hybridoma technology for MAB production against specific mycotoxins.
- Selection of MABs for sensitivity, specificity, and reliability.
- Application of MABs in immunoassay techniques like ELISA and immunoaffinity chromatography.
Main Results:
- MABs provide sensitive detection of mycotoxins, with lower limits in the ng/ml range.
- Immunoassays using MABs simplify sample preparation (solvent extraction and dilution).
- These methods successfully determine mycotoxins in diverse matrices like maize, peanuts, and milk.
Conclusions:
- Monoclonal antibodies are valuable tools for rapid and sensitive mycotoxin detection.
- Immunoassay techniques utilizing MABs enhance efficiency and accuracy in food safety analysis.
- MABs facilitate the monitoring of mycotoxin contamination in agricultural products and animal tissues.