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Yeast RNA polymerase II initiation factor e: isolation and identification as the functional counterpart of human
H Tschochner1, M H Sayre, P M Flanagan
1Department of Cell Biology, Fairchild Center, Stanford University School of Medicine, CA 94305.
Abstract:
Yeast RNA polymerase II initiation factor e was purified to homogeneity and identified by biochemical criteria as the counterpart of human transcription factor IIB. Factor e was essential for initiation of transcription from yeast and mammalian promoters in a reconstituted yeast transcription system. Activity resided in a single polypeptide of approximately 41 kDa, identified by peptide sequence analysis as the product of the SUA7 gene. Factor e interacted specifically with RNA polymerase II, consistent with a proposed role in determining the start site of transcription.