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Surface display of antibodies
M Little1, F Breitling, B Micheel
1Research Group Recombinant Antibodies, German Cancer Research Center, FSP4/0445, 69120 Heidelberg, Germany.
Biotechnology Advances
|January 1, 1994
Summary
Antibody surface display systems enable efficient screening of large antibody libraries. Phage display, particularly using the pIII protein, is a key method for selecting specific antibody clones from diverse gene pools.
Area of Science:
- Biotechnology
- Molecular Biology
- Immunology
Background:
- Screening large antibody libraries requires high-efficiency systems.
- Antibody display on microbial surfaces mimics natural immune system processes.
- Existing methods include phage and bacterial display systems.
Purpose of the Study:
- To review various antibody surface display systems.
- To discuss screening strategies for antibody libraries.
- To compare bacterial production of antibody fragments.
Main Methods:
- Displaying antibodies on the surface of microorganisms (e.g., phage, bacteria).
- Utilizing coat proteins (pIII, pVIII) or outer membrane components (OmpA, IgA protease) as carriers.
- Screening libraries generated from lymphocyte repertoires and gene synthesis.
Main Results:
- Phage display using the pIII protein is a routinely used system for antibody library screening.
- Various systems exist for displaying antibodies on microbial surfaces.
- Comparison of bacterial production yields for Fabs and single-chain variable fragments (scFv) was performed.
Conclusions:
- Antibody surface display systems are crucial for efficient library screening.
- Phage display offers a robust method for antibody discovery.
- The choice of system impacts antibody fragment production efficiency.