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Validation of the multiplex kit genRESMPX-2 for forensic casework analysis
A Junge1, T Lederer, G Braunschweiger
1Institute of Legal Medicine, Rheinische Friedrich-Wilhelms-University Bonn, Stiftsplatz 12, 53111, Bonn, Germany. a.junge@uni-bonn.de
International Journal of Legal Medicine
|October 11, 2003
Summary
The genRESMPX-2 PCR multiplex system reliably analyzes DNA, even with limited or degraded samples. This system is suitable for forensic casework, including mixed DNA samples.
Area of Science:
- Forensic Science
- Molecular Biology
- Genetics
Background:
- The German DNA database, established in 1998 by the Federal Criminal Office (BKA), utilizes specific Short Tandem Repeat (STR) systems.
- Commercial multiplex PCR systems are crucial for efficient DNA profiling in forensic investigations.
Purpose of the Study:
- To validate the genRESMPX-2 PCR multiplex system for forensic DNA analysis.
- To assess the system's performance with varying DNA quantities and sample types.
Main Methods:
- Validation using the commercially available genRESMPX-2 PCR multiplex system.
- Testing with varying amounts of template DNA (100 pg to 200 pg).
- Analysis of mixed DNA samples at different ratios (e.g., 1:5 to 1:20).
Main Results:
- The genRESMPX-2 system successfully generated complete DNA profiles from 100 pg to 200 pg of template DNA.
- Mixed samples were clearly distinguishable at ratios from 1:5 (ACTBP2) to 1:20 (VWA, FGA).
- The system demonstrated sensitivity, reliability, and robustness across various forensic materials and conditions.
Conclusions:
- The genRESMPX-2 kit is a sensitive, reliable, and robust multiplex system for forensic casework.
- It is effective for analyzing samples with limited amounts or degraded DNA.
- The system is suitable for use with the STR loci present in the German DNA database.