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Updated: Aug 19, 2026

Isolation of Human Umbilical Vein Endothelial Cells (HUVEC)
Published on: April 28, 2007
[Effect of human telomerase reverse transcriptase mRNA introduction on the growth of human umbilicus vein endothelial
Yi Qu1, Zheng-rong Wang, Xiang Huang
1Laboratory of Medical Molecular Biology, School of Basic and Forensic Medicine, Sichuan University, Chengdu, Sichuan, P. R. China 610041. quyi712002@yahoo.com.cn
Objective:
To elongate the proliferation life-span of human umbilicus vein endothelial cell (HUVEC).
Methods:
We synthesized the human telomerase reverse transcriptase mRNA (hTERT mRNA) by in vitro transcription, then transferred the hTERT mRNA into HUVEC in quicent stage by lipofect introduction.
Results:
Telomerase expressed transiently in HUVEC, and the cell life-span was elongated for 7 population doublings.
Conclusion:
Telomerase can be reconstructed controllably and transiently in HUVEC by hTERT mRNA introduction, this method has the potential to be used to elongate the lifespan of cells cultured in vitro.
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