Related Experiment Video

Updated: Mar 23, 2026

Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira
14:49

Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira

Published on: August 14, 2021

5.8K

RNA interference silences Microplitis demolitor bracovirus genes and implicates glc1.8 in disruption of adhesion in

Markus Beck1, Michael R Strand

  • 1Department of Entomology, University of Georgia, Athens, GA 30602, USA.

Virology
|October 14, 2003
PubMed

Insights

RNA interference (RNAi) effectively silenced polydnavirus (PDV) genes in insect cells, revealing glc1.8

Area of Science:

  • Virology
  • Insect Pathology
  • Molecular Biology

Background:

  • Polydnaviruses (PDVs) are ds-DNA viruses symbiotically linked with parasitoid wasps.
  • PDVs are injected into hosts, causing physiological changes like immunosuppression.
  • Genes responsible for PDV-induced host alterations are poorly understood due to difficulties in creating viral mutants.

Purpose of the Study:

  • To investigate the function of Microplitis demolitor bracovirus (MdBV) genes glc1.8 and egf1.0 using RNA interference (RNAi).
  • To analyze the impact of MdBV infection on High Five insect cells, specifically their adhesion properties.
  • To determine the role of glc1.8 and egf1.0 in MdBV-induced cellular responses.

Main Methods:

  • Utilized RNA interference (RNAi) to specifically silence glc1.8 and egf1.0 genes in High Five cells.
  • Infected High Five cells with MdBV and assessed their adhesion to culture plates.
  • Employed monoclonal antibody (mAb) markers to characterize High Five cells and their relation to lepidopteran hemocytes.

Main Results:

  • MdBV infection blocked High Five cell adhesion, mimicking effects on native hemocytes.
  • dsRNA targeting glc1.8 successfully silenced its expression and restored cell adhesion.
  • dsRNA targeting egf1.0 silenced its expression but did not affect cell adhesion.

Conclusions:

  • RNAi is a potent tool for studying PDV gene function.
  • The glc1.8 gene product is involved in MdBV-induced disruption of cell adhesion.
  • Further research can utilize RNAi to explore other PDV genes and their roles in host manipulation.

More Related Videos

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells15:29

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells

Published on: August 5, 2016

8.7K
Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus11:28

Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus

Published on: October 7, 2011

11.6K

Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
8.2K
Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
3.8K
GPCRs Regulate Adenylyl Cylase Activity01:09

GPCRs Regulate Adenylyl Cylase Activity

Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...
8.2K
Immunoglobulin-like Cell Adhesion Molecules01:31

Immunoglobulin-like Cell Adhesion Molecules

Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
4.5K
Exon Recombination02:32

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon...
4.3K
Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
14.0K