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Elevated expression of caveolin-1 at protein and mRNA level in human cirrhotic liver: relation with nitric oxide

Hiroaki Yokomori1, Masaya Oda, Kazunori Yoshimura

  • 1Department of Internal Medicine, Kitasato Institute Medical Center Hospital, 121-1 Arai, Kitamoto 364-8501, Japan.

Journal of Gastroenterology
|October 18, 2003
PubMed
Abstract

Insights

Enhanced caveolin-1 expression in cirrhotic liver may significantly reduce nitric oxide (NO) activity. This study investigated caveolin and eNOS levels in liver disease patients.

Area of Science:

  • Hepatology
  • Molecular Biology
  • Biochemistry

Background:

  • Caveolin binds endothelial nitric oxide synthase (eNOS), inhibiting its activity.
  • Caveolae are cellular structures whose principal protein is caveolin.

Purpose of the Study:

  • To examine caveolin and eNOS expression at protein and mRNA levels.
  • To compare expression in hepatocellular carcinoma and hepatitis C-related cirrhosis patients versus controls.

Main Methods:

  • Western blotting using specific antibodies for eNOS, caveolin-1, and calmodulin.
  • In situ hybridization (ISH) with peptide nucleic acid probes for eNOS and caveolin-1 mRNA.

Main Results:

  • Marked overexpression of caveolin-1 protein and mRNA in cirrhotic liver tissue.
  • eNOS protein and mRNA expression showed minimal changes in cirrhosis.
  • Calmodulin protein levels were reduced in cirrhotic liver.

Conclusions:

  • Increased caveolin-1 expression correlates with reduced NO catalytic activity in cirrhosis.
  • Caveolin-1 may play a role in the pathophysiology of liver cirrhosis.

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