Related Experiment Videos
Phosphono peptide nucleic acids with a constrained hydroxyproline-based backbone
Vladimir A Efimov1, Valeryi N Klykov, Oksana G Chakhmakhcheva
1Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia. eva@mail.ibch.ru
Nucleosides, Nucleotides & Nucleic Acids
|October 21, 2003
Abstract:
DNA mimics representing negatively charged analogues of peptide nucleic acids (PNAs), particularly hetero-oligomers constructed from alternating phosphono-PNA residues (pPNA) and monomers on the base of trans-4-hydroxy-L-proline (HypNA) as well as mimics composed of phosphono-HypNA monomers (pHypNA) were tested in a set of in vitro and in vivo assays, and they demonstrated a high potential for the use in nucleic acid based diagnostic, isolation of nucleic acids and antisense experiments.