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Novel biotin phosphoramidites with super-long tethering arms
A M Morocho1, V N Karamyshev, O V Shcherbinina
1Fidelity Systems, Inc., Gaithersburg, Maryland 20879, USA.
Nucleosides, Nucleotides & Nucleic Acids
|October 21, 2003
Abstract:
A number of novel biotin phosphoramidites, possessing exceptionally long and uncharged tethering arms, were synthesized from methoxyoxalamido (MOX) and succinimido (SUC) precursors. Included among these monomers is a uridine derivative with the biotin moiety attached through the 2'-position. Some of these phosphoramidites were used to make 5'-biotinylated primers, which were applied in direct sequencing of genomic DNA and capture of Sanger fragment pools.

