Related Experiment Videos
Conventional and micellar liquid chromatography method development for danazol and validation in capsules
R Gonzalo-Lumbreras1, R Izquierdo-Hornillos
1Department of Analytical Chemistry, Faculty of Chemistry, Universidad Complutense, Ciudad Universitaria, Madrid, Spain.
Journal of Pharmaceutical and Biomedical Analysis
|October 21, 2003
Summary
Two liquid chromatography methods were developed to determine danazol (DZ) in capsules. Both conventional and micellar liquid chromatography (MLC) methods offer accurate and precise quantification of danazol.
Area of Science:
- Analytical Chemistry
- Pharmaceutical Analysis
Background:
- Danazol (DZ) is a medication used to treat endometriosis and other conditions.
- Accurate quantification of danazol in pharmaceutical formulations is crucial for quality control.
Purpose of the Study:
- To develop and validate two isocratic liquid chromatographic methods for danazol determination in capsules.
- To compare the performance of conventional liquid chromatography (CLC) and micellar liquid chromatography (MLC) for danazol analysis.
Main Methods:
- Development and validation of two isocratic liquid chromatographic methods: CLC and MLC.
- Utilized canrenone (CAN) as an internal standard.
- Employed UV absorbance detection at 280 nm.
- Sample preparation involved a simple extraction procedure prior to analysis.
Main Results:
- Both CLC and MLC methods achieved baseline separation of danazol.
- Accuracy tests showed high recoveries (99 ± 10% for CLC, 101 ± 8% for MLC).
- Repeatability and intermediate precision were below 5% R.S.D. for both methods.
- Detection limits were 2.4 ng/g for CLC and 3.0 ng/g for MLC.
Conclusions:
- Both developed CLC and MLC methods are suitable for the accurate and precise determination of danazol in capsules.
- MLC offers an alternative chromatographic technique for danazol analysis with comparable performance to CLC.