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Improved production and purification of interferon-alpha-4a using a T7 RNA polymerase expression system

G J Waine¹, B D Wines, L Wang

¹Department of Biochemistry, Monash University, Clayton, Victoria, Australia.

Biochemistry International

|October 1, 1992

Abstract:

An improved method for the synthesis and purification of human interferon-alpha-4a is presented. Interferon-alpha-4a was prepared using a T7 RNA polymerase expression system, where it was expressed from the vector pET-3a. Biologically active interferon-alpha-4a was isolated from the E. coli cells and purified using protamine sulphate precipitation, anion-exchange chromatography and affinity chromatography utilising a monoclonal antibody specific for human interferons-alpha.

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