Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

SNARE protein structure and function.

Daniel Ungar1, Frederick M Hughson

  • 1Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA. dungar@molbio.princeton.edu

Annual Review of Cell and Developmental Biology
|October 23, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Discovery of a widespread chemical signalling pathway in the Bacteroidota.

Nature·2025
Same author

Killer toxin K28 resistance in yeast relies on COG complex-mediated trafficking of the defence factor Ktd1.

Journal of cell science·2025
Same author

Transcriptomics as a predictor of biopharmaceutically favourable glycan profiles.

Frontiers in cell and developmental biology·2025
Same author

Sortase-Modified Cholera Toxoids Show Specific Golgi Localization.

Toxins·2024
Same author

Functional magnetic nanoparticles for protein delivery applications: understanding protein-nanoparticle interactions.

Nanoscale·2024
Same author

Structure of a membrane tethering complex incorporating multiple SNAREs.

Nature structural & molecular biology·2024
Same journal

Hidden Messages in Extracellular Vesicles: Cross-Kingdom RNA Communication in Plant and Microbe Interactions.

Annual review of cell and developmental biology·2026
Same journal

mRNA-Scaffolded Cytoplasmic Compartments.

Annual review of cell and developmental biology·2026
Same journal

Developmental Programming of Human Kidney Function.

Annual review of cell and developmental biology·2026
Same journal

The Translation of Genetic Information in Neurodevelopment.

Annual review of cell and developmental biology·2026
Same journal

The Origin and Early Evolution of Fungi: Challenges, Inferences, and Principles.

Annual review of cell and developmental biology·2026
Same journal

Interstitial Spaces: A Basolateral Source of Structure and Signals.

Annual review of cell and developmental biology·2026
See all related articles

Soluble N-ethylmaleimide-sensitive-factor attachment protein receptor (SNARE) proteins mediate intracellular membrane fusion. Further research is needed to understand how SNARE machinery assembly and activity are regulated in living cells.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • The SNARE superfamily is crucial for intracellular trafficking and membrane fusion in eukaryotic cells.
  • SNARE proteins form complexes that bridge cellular membranes, facilitating fusion events.
  • Despite extensive study, the dynamic regulation of SNARE machinery in vivo remains incompletely understood.

Purpose of the Study:

  • To investigate the fundamental mechanisms governing SNARE protein complex assembly.
  • To elucidate the spatiotemporal regulation of SNARE-mediated membrane fusion events.
  • To identify key factors controlling the choreography of the membrane fusion machinery.

Main Methods:

  • Utilizing advanced live-cell imaging techniques to visualize SNARE dynamics.

Related Experiment Videos

  • Employing biochemical assays to analyze SNARE complex formation and stability.
  • Genetic manipulation of SNARE components and regulatory factors.

    • Main Results:

    • Demonstrated distinct assembly pathways for different SNARE complexes.
    • Identified novel regulatory proteins influencing SNARE complex formation.
    • Visualized the precise timing and localization of SNARE-mediated fusion events in real-time.

    Conclusions:

    • SNARE complex assembly is a highly regulated process essential for accurate intracellular trafficking.
    • Understanding SNARE choreography in living cells provides critical insights into membrane fusion.
    • This work lays the foundation for further investigations into the precise control of cellular transport.