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Infrared multiphoton dissociation with a hollow fiber waveguide
Jared J Drader1, James C Hannis, Steven A Hofstadler
1Ibis Therapeutics, Isis Pharmaceuticals, 2292 Faraday Avenue, Carlsbad, California 92008, USA.
Analytical Chemistry
|October 24, 2003
Summary
A new method uses a hollow fiber waveguide to deliver infrared radiation for infrared multiphoton dissociation (IRMPD) of ions in mass spectrometry. This technique efficiently fragments large molecules like oligonucleotides and proteins.
Area of Science:
- Analytical Chemistry
- Physical Chemistry
- Spectroscopy
Background:
- Infrared multiphoton dissociation (IRMPD) is a key technique for ion fragmentation in mass spectrometry.
- Delivering infrared (IR) radiation efficiently into ion traps or reservoirs can be challenging.
Purpose of the Study:
- To present a novel scheme for IRMPD using a hollow fiber waveguide (HFWG).
- To demonstrate efficient dissociation of oligonucleotide and protein ions using this HFWG-based IRMPD method.
Main Methods:
- A hollow fiber waveguide (HFWG) was employed to transmit 10.6-microm IR laser radiation into the ion storage region of a mass spectrometer.
- The IR laser beam was focused into a vacuum-sealed HFWG using a simple optical setup.
- IRMPD was performed on an ESI-FTICR instrument and a quadrupole ion trap.
Main Results:
- High power density IR radiation was delivered to ions via the HFWG due to its small internal diameter and high transfer efficiency.
- Efficient IRMPD of a 12-mer oligonucleotide and the protein melittin was achieved.
- Dissociation was demonstrated in both external ion reservoirs and quadrupole ion traps.
Conclusions:
- The HFWG is an effective tool for delivering IR radiation for IRMPD in mass spectrometry.
- This method enables efficient fragmentation of complex biomolecules, including oligonucleotides and proteins.
- The presented scheme offers a practical approach for enhancing IRMPD capabilities in various mass spectrometry instruments.