Antioxidant capacity of silica hydride: a combinational photosensitization and fluorescence detection assay

Utilizing a novel combinational technique incorporating spectrafluorometry and photosensitization, this analysis determined cell viability and cytotoxicity through the introduction of reactive oxygen species and measurement of plasma membrane integrity. Chinese hamster ovary and mouse hybridoma cells were treated with silica hydride after being photosensitized with singlet oxygen, hydroxyl/superoxide, and hydroxyl reactive oxygen species through the use of rose Bengal diacetate, malachite green, and N,N'-bis(2-hydroperoxy-2-methoxyethyl)-1,4,5,8-naphthaldiimide, respectively. The analysis resulted in an easy and effective method for quantifying reactive oxygen species reduction and characterized the radical reduction efficacy of silica hydride at 97% (+/- 0.68%, sigma = 0.84) against singlet oxygen species and over 87% (+/- 0.56%, sigma = 0.70) for the combination of hydroxyl and superoxide reactive species, and 98% (+/- 0.37%, sigma = 0.47) effective for hydroxyl radical species. Nontreated photosensitized controls showed less than 1% viability under the same conditions.