Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

U2AF modulates poly(A) length control by the poly(A)-limiting element.

Haidong Gu1, Daniel R Schoenberg

  • 1Department of Molecular and Cellular Biochemistry and the Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43210, USA.

Nucleic Acids Research
|October 25, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Associations of physical activity and sedentary behavior with chronic low back pain in middle-aged and older adults: a cross-sectional study.

Frontiers in public health·2026
Same author

Land conversion to cropland homogenizes variation in soil biota, gene assemblages, and ecological strategies on local and regional scales.

The ISME journal·2025
Same author

Organic fertilizers increase microbial community diversity and stability slowing down the transformation process of nutrient cycling.

Environmental microbiome·2025
Same author

Biodegradable microplastics show greater potential than conventional types in facilitating antibiotic resistance gene enrichment and transfer through viral communities.

Environment international·2025
Same author

Warming-induced unstable microbial community metabolically lowers straw-carbon sequestration in paddy soils.

Journal of advanced research·2025
Same author

Long-term warming offsets the beneficial effect of elevated CO<sub>2</sub> on mineral associated organic carbon in Mollisols.

The Science of the total environment·2025
Same journal

Correction to 'scSuperAnnotator: A platform for benchmarking comparison and visualizing automated cellular annotation methods for scRNA-seq data'.

Nucleic acids research·2026
Same journal

Correction to 'Differentiable partition function calculation for RNA'.

Nucleic acids research·2026
Same journal

Deployment of non-canonical splicing in tunicate genomes is mediated by divergent U2AF function and changing m6A modification in U1 and U6 snRNA.

Nucleic acids research·2026
Same journal

Bacillus subtilis DnaB forms multiple protein-protein interactions essential for DNA replication initiation.

Nucleic acids research·2026
Same journal

Multiple forms of protein-protein and DNA binding are exhibited by BrxC from the BREX phage restriction system.

Nucleic acids research·2026
Same journal

Biosynthesis of glycosylated 5-hydroxycytosine in the DNA of diverse viruses.

Nucleic acids research·2026
See all related articles

The poly(A)-limiting element (PLE) controls mRNA poly(A) tail length. U2AF65 protein binding to PLE is crucial for restricting poly(A) tail size.

Area of Science:

  • Molecular Biology
  • RNA Processing
  • Gene Expression Regulation

Background:

  • The poly(A)-limiting element (PLE) is a regulatory sequence in pre-mRNA.
  • PLE restricts poly(A) tail length to less than 20 nucleotides.
  • A 65 kDa protein was previously found to bind the functional PLE.

Purpose of the Study:

  • To identify the 65 kDa protein that binds the PLE.
  • To investigate the role of this protein in poly(A) tail length regulation.
  • To elucidate the mechanism by which U2AF65 modulates PLE function.

Main Methods:

  • Protein cross-linking assays to identify PLE-binding proteins.
  • RNase footprinting to determine binding specificity.
  • Protein purification and identification via chromatography.

Related Experiment Videos

  • Reporter gene assays with mutated PLE sequences and U2AF65 overexpression.

    • Main Results:

    • The 65 kDa protein was identified as the large subunit of U2 snRNP auxiliary factor (U2AF65).
    • U2AF65 selectively binds to pyrimidine-rich regions of the PLE.
    • Overexpression of U2AF65 led to heterogeneous poly(A) tails, an effect dependent on its RNA recognition motifs (RRMs).
    • A specific mutation in the PLE restored discrete poly(A) tail length in the presence of U2AF65.

    Conclusions:

    • U2AF65 plays a critical role in modulating the function of the poly(A)-limiting element.
    • U2AF65 likely facilitates the binding of other proteins to the PLE to regulate poly(A) tail length.
    • The C-terminal RRMs of U2AF65 are essential for its function in poly(A) tail length control.